Transgenic Expression of L-Gulono-y-lactone oxidase in Medaka (Oryzias latipes), a teleost fish that lacks this enzyme necessary for L-ascorbic acid biosynthesis.
Transfer of the gene for L-gulono-y-lactone oxidase, the missing enzyme in L-ascorbic acid biosynthesis in scurvy-prone animals, into medaka fish was successfully done. The plasmid for GLO cDNA was microinjected into the cytoplasm of fertilized eggs. Four male fish having the transgene in their germs cells came to maturity, and progeny derived from one of the fish possessed L-gulono-y-lactone oxidase activity.
Biochemical & Biophysical Research Communications, Volume 223, Issue 3, 25 June 1996, Pages 650-653